C-1 Mitochondrial Membrane Potential Detection Kit provides a quick and reliable method to detect the mitochondrial membrane potential changes in apoptotic cells using flow cytometry. The loss of mitochondrial membrane potential is a hallmark for apoptosis. 
Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. In healthy cells, the JC-1 accumulates in the mitochondrial to form J-aggregates, which become red fluorescence. In apoptotic cells, the JC-1 cannot accumulate within the mitochondria and remains in the cytoplasm in monomeric form to show green fluorescence.  
Features:

1. Simple workflow—add, incubate, and read format, no wash needed 
2. Flexibility—suitable for flow cytometry, fluorescence microscope, and plate reader 
3. Fast—results in as little as 30 minutes 

Component：1. JC-1(200×) 100μL×5
                      2. CCCP(10mM)    20μL
                      3. Ultrapure Water  90mL
                      4. JC-1 Stain Buffer(5×) 80mL

Storage： -20℃. Protect from light.

Shelf Life：Store at -20℃ for 1 year.