The Hoechst 33342 Staining Solution is a ready-to-use reagent for the identification of nucleated cells by flow cytometric analysis. Hoechst 33342 is widely used for enumeration of nucleated cells and for determination of DNA content in cell cycle analysis. Hoechst 33342 is also frequently used as a nuclear counterstain in fluorescence microscopy assays.
       Hoechst 33342 is a cell-permeable DNA stain that is excited by ultraviolet light and emits blue fluorescence at 460 to 490 nm. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. the staining intensity differs between different cell types,All nucleated cells are Hoechst positive.

1. Very effective at removing mycoplasma from infected cell cultures at a low concentration (0.5µg/ml)
2. Maintains cell viability
3. Suitable for use to prevent recontamination of the culture with the original mycoplasma
4. Very easy to use — simply incubate for a week after addition to mycoplasma-contaminated cell cultures
5. Sterility and low cytotoxicity
6. Can be used to prevent initial infection of cells in culture by mycoplasma
7. Compatible with most mammalian cell lines

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1. Counterstain—ideal for use alongside detection of specific targets with fluorescent antibodies for fluorescence microscopy or high-content screening (HCS)
2. Convenient—provided as an easy-to-use Hoechst dye solution 
3. Cellular imaging—cell-permeable dye is effective for fixed-cell and live-cell staining
4. Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells)

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Storage：-20℃. Protect from light.

Shelf Life：Store at -20℃ for 2 years.