Tris-glycine-SDS running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is usually used for both anode and cathode buffer. It is also used to block membranes with 5% non-fat dry milk for western blotting.
This product is a 5× concentrated solution, diluted to 1× working concentration before use: take 100ml of 5×Tris-glycine electrophoresis buffer, add distilled water or deionized water to make the volume up to 500ml and mix.
1. Ready to dilute — use distilled deionized water
2. Reduced preparation time — no reagents to weigh or filter
3. Quality-controlled reagent — guarantees reproducible results
3. SDS—0.5% (W/V)）
Shelf Life：Store at RT for 1 year.