Ultra-sensitive ECL chemiluminescent substrate is an ultra-sensitive, luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) at high sensitivity levels (low picogram to mid-femtogram). 

The ECL substrate is compatible with various membranes, blocking solutions and a wide range of antibody diluents, and with excellent performance, versatility and high cost performance, it can meet the needs of users for western blotting applications. This product offers superior signal and clear background.

Features:

1. Sensitivity—detection of femtogram protein 

2. Signal duration—up to six or eight hours
3. Stability—48 hours for working solution, one year for kit at 4℃
4. Compatibility—nitrocellulose and PVDF membranes
5. Recommended primary antibody concentration—1:1,000—1:5,000 dilution(1µg/mL stock solution)
6. Recommended secondary antibody concentration—1:20,000—1:100,000 dilution(1µg/mL stock solution)

Component：Reagent A—ECL 

                      Chemiluminescence

                      Substrate A Solution
                      Reagent B—ECL

                      Chemiluminescence

                      Substrate B Solution
Storage：4 ℃.

Shelf Life：Transported at RT, stored at 4°C away from light for 1 year