Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e. g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i. e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc. ) to generate highly specific reagents. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species

Features:

1:500 - 1:5,000 for immunohisto/cytochemistry
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates

1:10,000 - 1:200,000 for Western blotting with ECL substrates
This product added an equal volume of glycerol for a final concentration of 50% for store at -20°C as a liquid. So the the stated protein concentration and dilution range were reduced by one-half.

Component：1. Antibody Concentration: 0.8 mg/ml
                       2. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
                       3. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
                       4. Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)

Storage： -20℃

Shelf Life：Store at -20℃ for 1 year.